Formalin fixed paraffin embedded (FFPE) tissue blocks are now widely used in all fields of study. The routine processing of tissues from post-mortem, surgery, and biopsy has led to a vast collection of specimens that represent various diseases and its different stages.
FFPE Tissue Blocks is a great commodity to the research and medical field as it is an inexpensive and easy way to store and archive Human and animal tissue specimens. It also preserves the integrity of the specimen for a long time and can therefore be used retrospectively in many studies which reduces the time needed to track down specific tissues that fit the criteria of the study.
FFPE allows the extraction of high quality nucleic acids and can be widely used for genomic biomarker discovery studies. A big part of biomarker research in tissue is centered on cancer. Just like any other types of disease, cancer tissue usually shows regional heterogeneity in cell preservation and cell type.
The four different extraction protocols compared with or without the use of proteinase K digestion are: Heat treatment, QIAamp DNA-blood-mini-kit extraction, EasyMAG NucliSens extraction, Gentra Capture0Column-kit extraction.
The acceleration of oxidative phosphorylation, aerobic glycolysis and its parallel pentose phosphate pathway make glucose metabolism a possible target for cancer therapies by distinguishing their metabolic profiles from non-cancerous cells.